Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1) (Center) antibody

Antigen: Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)

Synonyms: TM, EAT, MCL1L, MCL1S, Mcl-1, BCL2L3, MGC1839, MGC104264

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657627

Additional Information

Alternative name: MCL1

Gene ID: 4170

UniProt: NP_877495.1, NP_068779.1, Q07820

Immunogen: This MCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the Central region of human MCL1.

Isotype: Ig

Description: Other names: Induced myeloid leukemia cell differentiation protein Mcl-1,MCL1,BCL2L3

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This MCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-245 amino acids from the Central region of human MCL1.

Molecular Weight: 37337 DA

Comments:

Background: This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: MCL1 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Structure, Protein Modifications, Cell Cycle

Restrictions: For Research Use only

Images

MCL1 Antibody (Center) (ABIN657627) western blot analysis in Y79 cell line lysates (35ug/lane). This demonstrates the MCL1 antibody detected the MCL1 protein (arrow).

Publications

Product: Domina, Vrana, Gregory et al.: "MCL1 is phosphorylated in the PEST region and stabilized upon ERK activation in viable cells, and at additional sites with cytotoxic okadaic acid or taxol." in: Oncogene, Vol. 23, Issue 31, pp. 5301-15, 2004 (PubMed).

Maurer, Charvet, Wagman et al.: "Glycogen synthase kinase-3 regulates mitochondrial outer membrane permeabilization and apoptosis by destabilization of MCL-1." in: Molecular cell, Vol. 21, Issue 6, pp. 749-60, 2006 (PubMed).

Shimada, Miyagawa, Kawashima et al.: "An approach based on a genome-wide association study reveals candidate loci for narcolepsy." in: Human genetics, Vol. 128, Issue 4, pp. 433-41, 2010 (PubMed).