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Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 63-92)

Details for Product anti-NCK1 Antibody No. ABIN657648, Supplier: Log in to see
Antigen
  • NCK1
  • NCK
  • NCKalpha
  • nck-1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK1
  • LOC100347965
  • LOC100358703
  • Nck1
Alternatives
anti-Human Cytoplasmic Protein NCK1 antibody for Immunoprecipitation
Epitope
AA 63-92, N-Term
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24
14
5
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
122
30
25
8
4
4
2
2
2
2
2
2
1
1
1
1
1
1
Host
Rabbit
73
48
1
Clonality (Clone)
Polyclonal ()
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
5
4
4
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
119
75
47
15
12
10
7
4
3
2
1
1
1
Options
Supplier
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Immunogen This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63-92 AA from the N-terminal region of human NCK1.
Clone RB33697
Isotype Ig
Specificity This NCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-92 amino acids from the N-terminal region of human NCK1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Synonyms: Cytoplasmic protein NCK1,NCK1,NCK
Molecular Weight 42864 DA
Gene ID 4690
NCBI Accession NP_006144, NP_001177725
UniProt P16333
Research Area Translation Factors, Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cell Structure
Pathways TCR Signaling, Regulation of Actin Filament Polymerization
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment NCK1 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Cytoplasmic Protein NCK1 antibody (NCK Adaptor Protein 1) (AA 63-92) (ABIN657648) Western blot analysis of NCK1 (arrow) using rabbit polyclonal NCK1 Antibody (N-term) ...
Background publications Barda-Saad, Shirasu, Pauker, Hassan, Perl, Balbo, Yamaguchi, Houtman, Appella, Schuck, Samelson: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010 (PubMed).

Gehmlich, Hayess, Legler, Haebel, Van der Ven, Ehler, Fürst: "Ponsin interacts with Nck adapter proteins: implications for a role in cytoskeletal remodelling during differentiation of skeletal muscle cells." in: European journal of cell biology, Vol. 89, Issue 5, pp. 351-64, 2010 (PubMed).

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010 (PubMed).