Splicing Factor 3b, Subunit 1, 155kDa (SF3B1) (N-Term) antibody

Antigen: Splicing Factor 3b, Subunit 1, 155kDa (SF3B1)

Synonyms: PRP10, PRPF10, SAP155, SF3b155, TA-8, Prp10, Targ4, 155kDa, AA409119, 2810001M05Rik, prp10, hsh155, prpf10, sap155, MGC115390, SF3B1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657676

Additional Information

Alternative name: SF3B1

Gene ID: 23451

UniProt: NP_036565.2, O75533

Immunogen: This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the N-terminal region of human SF3B1.

Isotype: Ig

Description: Other names: Splicing factor 3B subunit 1,SF3B1,SAP155

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the N-terminal region of human SF3B1.

Molecular Weight: 145830 DA

Comments:

Background: This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: SF3B1 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure

Restrictions: For Research Use only

Images

SF3B1 Antibody (N-term) (ABIN657676) western blot analysis in CEM cell line lysates (35ug/lane). This demonstrates the SF3B1 antibody detected the SF3B1 protein (arrow).

Publications

Product: Pessa, Will, Meng et al.: "Minor spliceosome components are predominantly localized in the nucleus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 25, pp. 8655-60, 2008 (PubMed).

Tanuma, Kim, Beullens et al.: "Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155)." in: The Journal of biological chemistry, Vol. 283, Issue 51, pp. 35805-14, 2008 (PubMed).

Corsini, Hothorn, Stier et al.: "Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60." in: The Journal of biological chemistry, Vol. 284, Issue 1, pp. 630-9, 2008 (PubMed).