Vav 1 Oncogene (VAV1) (Center) antibody

Antigen: Vav 1 Oncogene (VAV1)

Synonyms: VAV, Vav, vav-T, vav1, MGC80607, VAV1

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657774

Additional Information

Alternative name: VAV1

Gene ID: 7409

UniProt: NP_005419.2, P15498

Immunogen: This VAV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-466 amino acids from the Central region of human VAV1.

Isotype: Ig

Description: Other names: Proto-oncogene vav,VAV1,VAV

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This VAV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 436-466 amino acids from the Central region of human VAV1.

Molecular Weight: 98314 DA

Comments:

Background: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: VAV1 Antibody (Center) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Signaling, Phospho-specific antibodies, Cell Signaling, Protein Modifications, Cancer, Cell Structure

Restrictions: For Research Use only

Images

VAV1 Antibody (Center) (ABIN657774) western blot analysis in CEM cell line lysates (35ug/lane). This demonstrates the VAV1 antibody detected the VAV1 protein (arrow).

Publications

Product: Davila, Froeling, Tan et al.: "New genetic associations detected in a host response study to hepatitis B vaccine." in: Genes and immunity, Vol. 11, Issue 3, pp. 232-8, 2010 (PubMed).

Barda-Saad, Shirasu, Pauker et al.: "Cooperative interactions at the SLP-76 complex are critical for actin polymerization." in: The EMBO journal, Vol. 29, Issue 14, pp. 2315-28, 2010 (PubMed).