Topoisomerase (DNA) I (TOP1) (N-Term) antibody

Antigen: Topoisomerase (DNA) I (TOP1)

Synonyms: TOPI, Top-1, AI467334, D130064I21Rik, TOP1, TopI, Topo I, TopoI, dTopoI, l(1)G0134, l(1)G0201, l(1)G0229, l(1)G0278, l(1)top1, DmelCG6146, CG6146, Ab2-086

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657818

Additional Information

Alternative name: TOP1

Gene ID: 7150

UniProt: NP_003277.1, P11387

Immunogen: This TOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-31 amino acids from the N-terminal region of human TOP1.

Isotype: Ig

Description: Other names: DNA topoisomerase 1,TOP1,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This TOP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-31 amino acids from the N-terminal region of human TOP1.

Molecular Weight: 90726 DA

Comments:

Background: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: TOP1 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Ubiquitin-related antibodies, Signaling, Phospho-specific antibodies, Protein Modifications, Cancer, Transcription Factors, Chromatin, Cell Structure

Restrictions: For Research Use only

Images

TOP1 Antibody (N-term) (ABIN657818) western blot analysis in NCI-H460 cell line lysates (35ug/lane). This demonstrates the TOP1 antibody detected the TOP1 protein (arrow).

Publications

Product: Reinhold, Mergny, Liu et al.: "Exon array analyses across the NCI-60 reveal potential regulation of TOP1 by transcription pausing at guanosine quartets in the first intron." in: Cancer research, Vol. 70, Issue 6, pp. 2191-203, 2010 (PubMed).

Kjeldsen, Tordrup, Hübner et al.: "Topoisomerase I deficiency results in chromosomal alterations in cervical cancer cells." in: Anticancer research, Vol. 30, Issue 9, pp. 3257-65, 2010 (PubMed).