Laminin, beta 1 (LAMB1) (C-Term) antibody

Antigen: Laminin, beta 1 (LAMB1)

Synonyms: CLM, MGC142015, gup, fc20b05, sb:cb95, wu:fc20b05, Lamb1-1, LAMB1, lams, lamb2

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Please enquire

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657904

Additional Information

Alternative name: LAMB1

Gene ID: 3912

UniProt: NP_002282.2, P07942

Immunogen: This LAMB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1690-1720 amino acids from the C-terminal region of human LAMB1.

Isotype: Ig

Description: Other names: Laminin subunit beta-1,3912,

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This LAMB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1690-1720 amino acids from the C-terminal region of human LAMB1.

Molecular Weight: 198066 DA

Comments:

Background: Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the beta chain isoform laminin, beta 1. The beta 1 chain has 7 structurally distinct domains which it shares with other beta chain isomers. The C-terminal helical region containing domains I and II are separated by domain alpha, domains III and V contain several EGF-like repeats, and domains IV and VI have a globular conformation. Laminin, beta 1 is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin 1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. A sequence in the beta 1 chain that is involved in cell attachment, chemotaxis, and binding to the laminin receptor was identified and shown to have the capacity to inhibit metastasis.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: LAMB1 Antibody (C-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Cell Signaling, Cell Structure, Protein Modifications

Restrictions: For Research Use only

Images

LAMB1 Antibody (C-term) (ABIN657904) western blot analysis in mouse lung tissue lysates (35ug/lane). This demonstrates the LAMB1 antibody detected the LAMB1 protein (arrow).

Publications

Product: Rooney, Gurpur, Burkin: "Laminin-111 protein therapy prevents muscle disease in the mdx mouse model for Duchenne muscular dystrophy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 19, pp. 7991-6, 2009 (PubMed).

Wheeler, Metter, Tanaka et al.: "Sequential use of transcriptional profiling, expression quantitative trait mapping, and gene association implicates MMP20 in human kidney aging." in: PLoS genetics, Vol. 5, Issue 10, pp. e1000685, 2009 (PubMed).

Joslyn, Ravindranathan, Brush et al.: "Human variation in alcohol response is influenced by variation in neuronal signaling genes." in: Alcoholism, clinical and experimental research, Vol. 34, Issue 5, pp. 800-12, 2010 (PubMed).