HES1 (N-Term) antibody

Antigen: HES1

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN657944

Additional Information

Gene ID: 3280

UniProt: NP_005515.1, Q14469

Immunogen: This HES1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human HES1.

Isotype: Ig

Description: Other names: Transcription factor HES-1,3280,BHLHB39, HL, HRY

Characteristics: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HES1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human HES1.

Molecular Weight: 29541 DA

Comments:

Background: This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:100~500

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Storage: HES1 Antibody (N-term) can be refrigerated at 2-8°,C for up to 6 months. For long term storage, place the at -20°,C.

Research Area: Translation Factors, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Signaling, Cell Structure

Restrictions: For Research Use only

Images

HES1 Antibody (N-term) (ABIN657944) western blot analysis in U251 cell line lysates (35ug/lane). This demonstrates the HES1 antibody detected the HES1 protein (arrow).

Publications

Product: Sang, Roberts, Coller: "Hijacking HES1: how tumors co-opt the anti-differentiation strategies of quiescent cells." in: Trends in molecular medicine, Vol. 16, Issue 1, pp. 17-26, 2010 (PubMed).

Wang, Fu, Chen et al.: "The expressions of bHLH gene HES1 and HES5 in advanced ovarian serous adenocarcinomas and their prognostic significance: a retrospective clinical study." in: Journal of cancer research and clinical oncology, Vol. 136, Issue 7, pp. 989-96, 2010 (PubMed).

Espinosa, Cathelin, DAltri et al.: "The Notch/Hes1 pathway sustains NF-κB activation through CYLD repression in T cell leukemia." in: Cancer cell, Vol. 18, Issue 3, pp. 268-81, 2010 (PubMed).