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RAT SERPH (AA 222-251), (Center) antibody

Details for Product No. ABIN658998
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Antigen
Epitope
AA 222-251, Center
(1), (1)
Reactivity
Human, Rat (Rattus)
(1), (1)
Host
Mouse
(1)
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(1), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN658998
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Immunogen This Rat Serpinh1 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 222-251 AA from the Central region of rat Serpinh1.
Clone 134CT3-1-1
Isotype IgG1
Specificity This RAT Serpinh1 antibody is generated from mouse immunized with a KLH conjugated synthetic peptide selected from the Central region of RAT Serpinh1.
Predicted Reactivity Cow (Bovine),Mouse (Murine)
Purification This antibody is purified through a protein G column, followed by dialysis against PBS.
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 46590 DA
Gene ID 12406
UniProt P19324
Application Notes WB = 1:100-200, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 0.35 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-RAT SERPH (AA 222-251), (Center) antibody Western blot analysis of RAT SERPH Antibody (Center) in rat heart tissue lysates (35ug/lane).SERPH (arrow) was detected using the purified Pab.(1:100)
anti-RAT SERPH (AA 222-251), (Center) antibody (2) RAT SERPH Monoclonal Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RAT SERPH Monoclonal Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Background publications Ishida, Kubota, Yamamoto et al.: "Type I collagen in Hsp47-null cells is aggregated in endoplasmic reticulum and deficient in N-propeptide processing and fibrillogenesis." in: Molecular biology of the cell, Vol. 17, Issue 5, pp. 2346-55, 2006 (PubMed).

Wang, Li: "Adenovirus-mediated RNA interference against collagen-specific molecular chaperone 47-KDa heat shock protein suppresses scar formation on mouse wounds." in: Cell biology international, Vol. 32, Issue 5, pp. 484-93, 2008 (PubMed).

Zhou, Ma, Kong et al.: "Fog2 is critical for cardiac function and maintenance of coronary vasculature in the adult mouse heart." in: The Journal of clinical investigation, Vol. 119, Issue 6, pp. 1462-76, 2009 (PubMed).

Sansom, Griffiths, Faedo et al.: "The level of the transcription factor Pax6 is essential for controlling the balance between neural stem cell self-renewal and neurogenesis." in: PLoS genetics, Vol. 5, Issue 6, pp. e1000511, 2009 (PubMed).

Kakugawa, Mukae, Hishikawa et al.: "Localization of HSP47 mRNA in murine bleomycin-induced pulmonary fibrosis." in: Virchows Archiv : an international journal of pathology, Vol. 456, Issue 3, pp. 309-15, 2010 (PubMed).

Validation Images
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