Eukaryotic Translation Initiation Factor 4E Family Member 2 (EIF4E2) antibody

Antigen: Eukaryotic Translation Initiation Factor 4E Family Member 2 (EIF4E2)

Synonyms: EIF4E2, MGC152103, eif4e2, MGC158544, id:ibd1007, zgc:158544, MGC115622, DKFZp459H223

Clonality: Monoclonal

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN659116

Additional Information

Alternative name: EIF4E2

Gene ID: 9470

UniProt: O60573, NP_004837.1

Immunogen: This EIF4E2 monoclonal antibody is generated from mouse immunized with EIF4E2 recombinant protein.

Isotype: IgG1 kappa

Description: Other names: Eukaryotic translation initiation factor 4E type 2,EIF4E2,EIF4EL3

Characteristics: Mouse Monoclonal Antibody (Mab)

Specificity: This EIF4E2 monoclonal antibody is generated from mouse immunized with EIF4E2 recombinant protein.

Molecular Weight: 28362 DA

Comments:

Background: EIF4E2 recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Application Details

Application Notes: The suggested dilution is: ELISA~~1:1000 Western blotting~~ 1:10~250

Concentration: 0.25 mg/ml

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Translation Factors, Signaling, Protein Modifications, Chromatin, Cell Structure

Restrictions: For Research Use only

Publications

Product: Rosettani, Knapp, Vismara et al.: "Structures of the human eIF4E homologous protein, h4EHP, in its m7GTP-bound and unliganded forms." in: Journal of molecular biology, Vol. 368, Issue 3, pp. 691-705, 2007 (PubMed).

Zuberek, Kubacka, Jablonowska et al.: "Weak binding affinity of human 4EHP for mRNA cap analogs." in: RNA (New York, N.Y.), Vol. 13, Issue 5, pp. 691-7, 2007 (PubMed).

Venkatesan, Rual, Vazquez et al.: "An empirical framework for binary interactome mapping." in: Nature methods, Vol. 6, Issue 1, pp. 83-90, 2008 (PubMed).

Watkins, Rukazenkov: "Response to 'Clinical outcomes in NSCLC patients with EGFR mutations: pooled analysis'
(Paz-Ares et al., J Cell Mol Med. 2010; 14: 51-69)." in: Journal of cellular and molecular medicine, Vol. 14, Issue 11, pp. 2693-4, 2010 (PubMed).