DIO2 antibody (AA 101-200) (HRP)

Catalog No. ABIN702469

This Rabbit Polyclonal antibody specifically detects DIO2 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Mouse and Rat.

Quick Overview for DIO2 antibody (AA 101-200) (HRP) (ABIN702469)

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DIO2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-DIO2 Antibody (HRP)

Binding Specificity: AA 101-200

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DIO2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Alternative Name: DIO2

Background:

Synonyms: 5DII, D2, Deiodinase, iodothyronine, type II, DIOII, ITDI2, SelY, Thyroxine deiodinase, type II, TXDI2, Type 2 DI, Type II 5' deiodinase, Type II iodothyronine deiodinase.

Background: DIO2 belongs to the iodothyronine deiodinase family and is responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into T3 (3,5,3'-triiodothyronine). It is essential for providing the brain with appropriate levels of T3 during the critical period of development. DIO2 is expressed in heart, skeletal muscle, placenta, fetal brain and several regions of the adult brain. There are two named isoforms.

Gene ID: 1734

Pathways: Hormone Transport, Hormone Activity