DIO2 antibody (Internal Region)

Catalog No. ABIN190862

There is 1 publication for this product available. The Goat Polyclonal anti-DIO2 antibody is suitable to detect DIO2 in samples from Human and Mouse. It has been validated for ELISA, IHC, FACS and IF.

Quick Overview for DIO2 antibody (Internal Region) (ABIN190862)

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This DIO2 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Grade: Verified

Product Details anti-DIO2 Antibody

Binding Specificity: Internal Region

Purpose: DIO2

Sequence: EVKKHQNQED RC

Specificity: This antibody is expected to recognise both reported isoform a (NP_000784.2 and NP_054644.1) and isoform b (NP_001007024.1).

Cross-Reactivity: Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence EVKKHQNQEDRC, from the internal region of the protein sequence according to NP_000784.2, NP_054644.1, NP_001007024.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration: 2-3 μg/mL. Data obtained from customer.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentratio

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-DIO2 antibody (ABIN190862)

Ma, Brouwer, Wesseling, Raj, van der Want, Boddeke, Balasubramaniyan, Copray: "Multipotent stem cell factor UGS148 is a marker for tanycytes in the adult hypothalamus." in: Molecular and cellular neurosciences, Vol. 65, pp. 21-30, (2015) (PubMed).

Target Details for DIO2

Target: DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))

Alternative Name: DIO2

Background: DIO2, deiodinase, iodothyronine, type II , 5DII, D2, SelY, TXDI2 , thyroxine deiodinase, type II, type 2 iodothyronine deiodinase, type II iodothyronine deiodinaseN: type-II 5'deiodinase

Gene ID: 1734, 13371, 65162

NCBI Accession: NP_000784, NP_054644, NP_001007024

Pathways: Hormone Transport, Hormone Activity