JNK antibody (pThr183, pTyr185)

Catalog No. ABIN2177520

The Rabbit Polyclonal anti-JNK antibody (ABIN2177520) specifically detects JNK in WB, ELISA, IHC (p), IF (p), IF (cc) and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for JNK antibody (pThr183, pTyr185) (ABIN2177520)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-JNK Antibody

Binding Specificity: pThr183, pTyr185

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human MAPK9 around the phosphorylation site of Thr183+Tyr185

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: MAPK8 +

Background:

Synonyms: MAPK8/MAPK9phospho T183/Y185, Mapk9, C Jun kinase 2, C Jun N terminal kinase 2, JNK 2, JNK-2, JNK 55, JNK2 alpha, JNK2 beta, JNK2alpha, JNK2B, JNK2beta, JNK55, Jun kinase, MAP kinase 9, MAPK 9, Mitogen activated protein kinase 9, P54 alpha, P54aSAPK, PRKM9, SAPK alpha, SAPK1a, SAPKa, Stress activated protein kinase JNK2.

Background: phosphorylated at the Thr-Pro-Tyr phosphorylation motif instead of the characteristic MAP kinase Thr-Glu-Tyr motif. JNK2 (p54a, SAPK1a), along with JNK1 and JNK3, is thought to play an important role in nuclear signal transduction through its environmental stress activation and subsequent phosphorylation of the nuclear transcription factor p53.

Gene ID: 5601

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins