BCAR1 antibody (pTyr410) (HRP)

Catalog No. ABIN716000

The Rabbit Polyclonal anti-BCAR1 antibody is suitable to detect BCAR1 in samples from Rat. It has been validated for ELISA, IHC (p) and IHC (fro).

Quick Overview for BCAR1 antibody (pTyr410) (HRP) (ABIN716000)

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCAR1 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BCAR1 Antibody (HRP)

Binding Specificity: pTyr410

Specificity: Will recognize BCAR1(Tyr414) in mouse and BCAR1(Tyr508) in rat

Cross-Reactivity: Rat

Predicted Reactivity: Human,Mouse,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr410.

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BCAR1

Target: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Alternative Name: BCAR1

Background:

Synonyms: CAS, CAS1, CASS1, CRKAS, P130Cas, Breast cancer anti-estrogen resistance protein 1, CRK-associated substrate, Cas scaffolding protein family member 1, BCAR1

Background: Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.

Gene ID: 9564

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling