CK7 antibody (Biotin)
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Biotin (1)
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Application
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 Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)
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| Catalog no. |
ABIN733825 |
| Quantity |
100 µg (1mg/1ml) (Variants) |
| Price |
290.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 10 to 15 Business Days |
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Immunogen
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Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from the middle of mouse CK7
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Format
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Lyophilized
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Reconstitution
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Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
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Isotype
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IgG
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Description
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Synonym: Cytokeratin 7, CK 7, K7, Keratin 7, Keratin,type II cytoskeletal 7, KRT7, SCL.
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Molecular Weight
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54kDa
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Comments
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Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections but are also a useful tool in cytopathology and flow cytometric assays.
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Application Notes
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WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)
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Concentration
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1mg/1ml
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Purification
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Antibodies are purified by protein A and peptide affinity chromatography.
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Buffer
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0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
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Storage
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Ship at room temperature. Store at -20°C
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Restrictions
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For Research Use only
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Alternatives for antigen "CK7", type "Antibodies"
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Hosts
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Rabbit (39), Mouse (3)
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Reactivities
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Human (26), Mouse (Murine) (19), Rat (Rattus) (17), Chicken (1), Dog (Canine) (1), Rabbit (1)
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Applications
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Flow Cytometry (FACS) (37), Immunofluorescence (IF) (35), Western Blotting (WB) (8), ELISA (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (3), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Biotin (1)
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