PERK antibody
| Antigen |
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| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Flow Cytometry (FACS), Immunofluorescence (IF)
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| Catalog no. |
ABIN740715 |
| Quantity |
100 µg (1mg/1ml) |
| Price |
255.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from humna PERK C-terminus
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Format
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Lyophilized
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Reconstitution
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Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)
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Isotype
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IgG (Matching secondary antibodies)
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Description
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Synonym: DKFZp781H1925, EC 2.7.11.1, Eukaryotic translation initiation factor 2 alpha kinase 3, EIF2AK3, Heme regulated EIF2 alpha kinase, HRI, HsPEK, Pancreatic eIF2-alpha kinase, PEK, PRKR like endoplasmic reticulum kinase, WRS, PRKR-like endoplasmic reticulum kinase.
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Molecular Weight
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125kDa
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Comments
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PERK is a GCN2 Ser/Thr protein kinase which phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2) leading to its inactivation. This results in a reduction of translational initiation and repression of global protein synthesis. Expression of this Type I membrane protein is ubiquitous at low levels but greatest expression is seen in secretory and endocrine tissues. Defects in EIF2AK3 are the cause of Wolcott-Rallison syndrome (WRS) which is a rare autosomal recessive disorder.
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Application Notes
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WB (1:100-500), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)
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Concentration
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1mg/1ml
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Purification
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affinity purified by Protein A
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Buffer
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0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
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Storage
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Ship at room temperature. Store at -20°C
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Research Area
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Chromatin and Nuclear Signaling, DNA/RNA
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Restrictions
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For Research Use only
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Alternatives for antigen "PERK", type "Antibodies"
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Hosts
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Rabbit (20)
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Reactivities
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Human (20), Mouse (Murine) (18), Rat (Rattus) (18), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Pig (Porcine) (1), Rabbit (1)
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Applications
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Immunofluorescence (IF) (18), Flow Cytometry (FACS) (17), Western Blotting (WB) (3), ELISA (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (1), Immunohistochemistry (IHC) (1), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1), Immunoprecipitation (IP) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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