PRKAA1/PRKAA2 antibody (pThr172, pThr183) (HRP)

Catalog No. ABIN752377

This Rabbit Polyclonal antibody specifically detects PRKAA1/PRKAA2 in WB, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse, Rat, Pig and Sheep.

Quick Overview for PRKAA1/PRKAA2 antibody (pThr172, pThr183) (HRP) (ABIN752377)

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Reactivity: Human, Mouse, Rat, Pig, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1/PRKAA2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PRKAA1/PRKAA2 Antibody (HRP)

Binding Specificity: pThr172, pThr183

Cross-Reactivity: Human, Mouse, Pig, Rat, Sheep

Predicted Reactivity: Dog,Cow,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse AMPK alpha 2 around the phosphorylation site of Thr172

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PRKAA1/PRKAA2

Target: PRKAA1/PRKAA2 (AMPK1/AMPK2)

Alternative Name: AMPK alpha-1/2

Background:

Synonyms: PRKAA1phospho T172, AMPK alpha 1 + AMPK alpha 2 phospho T183/T172, 5 AMP activated protein kinase alpha 1catalytic subunit, 5 AMP activated protein kinase catalytic alpha 1 chain, 5' AMP activated protein kinase catalytic subunit alpha 1, AAPK1, acetyl CoA carboxylase kinase, AI194361, AI450832, AL024255, AMP -activate kinase alpha 1 subunit, AMP-activated protein kinase, catalytic, alpha -1, AMPK 1, AMPK alpha 1 chain, AMPK, AMPK1, AMPKa1, AMPKalpha1, C130083N04Rik, cb116, EC 2.7.11.1, HMG CoA reductase kinase,hormone sensitive lipase kinase, im:7154392, kinase AMPK alpha1, MGC33776, MGC57364, PRKAA 1, PRKAA1, Protein kinase AMP activated alpha 1 catalytic subunit, SNF1-like protein AMPK, wu:fa94C10.

Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

Gene ID: 5562