IDH1 antibody (Internal Region)

Catalog No. ABIN783970

This Goat Polyclonal antibody specifically detects IDH1 in WB and EIA. It exhibits reactivity toward Saccharomyces cerevisiae.

Quick Overview for IDH1 antibody (Internal Region) (ABIN783970)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Saccharomyces cerevisiae

Host: Goat

Clonality: Polyclonal

Conjugate: This IDH1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-IDH1 Antibody

Binding Specificity: Internal Region

Sequence: C-EPGSRHVGLD IKGQN

Specificity: Recognizes IDH1 (Yeast).

Cross-Reactivity (Details): Species reactivity (expected):Saccharomyces cerevisiae S288cSpecies reactivity (tested):S.cerevisiae

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the Internal region of the protein sequence according to NP_014361.1. Genename: IDH1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: IDH1

Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. IDH1 is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. IDH1 contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.Synonyms: Isocitrate dehydrogenase [NAD] subunit 1, Isocitric dehydrogenase, NAD(+)-specific ICDH, YNL037C, mitochondrial

Gene ID: 216

NCBI Accession: NP_014361

Pathways: Warburg Effect