IDH1 antibody (AA 116-143)

Catalog No. ABIN3031506

The Rabbit Polyclonal anti-IDH1 antibody (ABIN3031506) specifically detects IDH1 in WB, IHC, ELISA, FACS and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for IDH1 antibody (AA 116-143) (ABIN3031506)

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IDH1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF)

Product Details anti-IDH1 Antibody

Binding Specificity: AA 116-143

Cross-Reactivity (Details): Expected species reactivity: Rat, Bovine

Purification: Purified

Immunogen: A portion of amino acids 116-143 from the human protein was used as the immunogen for this IDH1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the IDH1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the IDH1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for IDH1

Target: IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))

Alternative Name: IDH1

Background: IDH1 belongs to two distinct subclasses. The protein is the NADP(+)-dependent Isocitrate Dehydrogenase found in the cytoplasm and peroxisomes. This protein contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [Wiki]

UniProt: O75874

Pathways: Warburg Effect