Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
Specificity
Specific for ~29k AQP2 protein phosphorylated at Ser269. This antibody also recognizes the glycosylated form of AQP2 at ~37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
Cross-Reactivity (Details)
Species reactivity (expected):Bovine, Canine, Mouse. Species reactivity (tested):Rat.
Purification
Affinity Chromatography.
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser269 of Rat Aquaporin 2.
Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, Serine-269 has been identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2 (Hoffert JD et al., 2008).Synonyms: ADH water channel, AQP-CD, AQP2, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct