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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pSer137) antibody

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Synonyms darpp32, darpp-32, Darpp-32, Darpp32, DARPP-32, DARPP32, AU040756
(42), (40), (18), (13), (6), (6), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Non Human Primates, Rat (Rattus)
(154), (117), (103), (19), (18), (17), (6), (6), (6), (3), (3), (2), (1)
(162), (13), (3), (2), (1)
(3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Western Blotting (WB)
(137), (57), (47), (31), (24), (19), (7), (5), (5), (2)
Pubmed 5 references available
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Quantity 0.1 mL
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser137 of Rat DARPP-32.
Isotype IgG
Specificity Specific for the ~32k DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by by preadsorption with the phospho-peptide used as antigen but not by the dephospho-peptide.
Cross-Reactivity (Details) Species reactivity (expected):Bovine, Canine, Chicken, Human, Mouse and non-Human Primates.
Species reactivity (tested):Rat.
Purification Affinity Chromatography.
Alternative Name DARPP32 (PPP1R1B Antibody Abstract)
Background DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Serine 137 is phosphorylated by casein kinase 1 and phosphorylation of this site is increased following acute administration of Prozac® (Svenningsson et al., 2002).Synonyms: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B
Gene ID 84152
NCBI Accession NP_001229393
Research Area Signaling
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pSer137) antibody (ABIN784599) Western blot of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-...
Background publications Lindskog, Svenningsson, Pozzi et al.: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Maldve, Zhang, Ferrani-Kile et al.: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).

Svenningsson, Tzavara, Witkin et al.: "Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3182-7, 2002 (PubMed).

Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

Fienberg, Hiroi, Mermelstein et al.: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." in: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, 1998 (PubMed).

Catalog No. ABIN784599
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