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Details for Product No. ABIN784599

Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B) (pSer137) antibody

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Antigen
Synonyms Darpp32, AU040756, DARPP-32, DARPP32, Darpp-32, darpp32, darpp-32
Epitope
»Alternatives pSer137
Reactivity
»Alternatives Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB)
Pubmed 5 references available
Catalog no. ABIN784599
Quantity 0.1 mL
Price
379.50 $   Plus shipping costs $45.00
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser137 of Rat DARPP-32.
Isotype IgG
Specificity Specific for the ~32k DARPP-32 protein phosphorylated at Ser137. Immunolabeling is blocked by by preadsorption with the phospho-peptide used as antigen but not by the dephospho-peptide.
Purification Affinity Chromatography.
Alternative Name DARPP32
Background DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons (Fienberg et al., 1998). The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA (Bibb et al., 1999). Phosphorylation of DARPP-32 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac® (Maldve et al., 2002, Lindskog et al., 2002, Svenningsson et al., 2002). Serine 137 is phosphorylated by casein kinase 1 and phosphorylation of this site is increased following acute administration of Prozac® (Svenningsson et al., 2002).
Alternate names: DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein, FLJ20940, PPP1R1B, Protein phosphatase 1 regulatory subunit 1B
Gene ID 84152
NCBI Accession NP_001229393
Research Area Signaling
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20°C.
Expiry Date 12 months
Background publications Fienberg, Hiroi, Mermelstein et al.: "DARPP-32: regulator of the efficacy of dopaminergic neurotransmission." in: Science (New York, N.Y.), Vol. 281, Issue 5378, pp. 838-42, 1998 (PubMed).

Bibb, Snyder, Nishi et al.: "Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons." in: Nature, Vol. 402, Issue 6762, pp. 669-71, 2000 (PubMed).

Svenningsson, Tzavara, Witkin et al.: "Involvement of striatal and extrastriatal DARPP-32 in biochemical and behavioral effects of fluoxetine (Prozac)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 5, pp. 3182-7, 2002 (PubMed).

Maldve, Zhang, Ferrani-Kile et al.: "DARPP-32 and regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens." in: Nature neuroscience, Vol. 5, Issue 7, pp. 641-8, 2002 (PubMed).

Lindskog, Svenningsson, Pozzi et al.: "Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine." in: Nature, Vol. 418, Issue 6899, pp. 774-8, 2002 (PubMed).

Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 1B (PPP1R1B)", type "Antibodies"
Hosts (125), (6), (2), (2)
Reactivities (114), (101), (88), (50), (48), (36), (36), (11), (4), (4), (2), (1), (1)
Applications (97), (45), (35), (30), (22), (21), (8), (6), (3), (2), (1), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (37), (28), (11), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
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