NKX6-1 antibody (N-Term)

Catalog No. ABIN784751

The Rabbit Polyclonal anti-NKX6-1 antibody has been validated for WB. It is suitable to detect NKX6-1 in samples from Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Cow and Zebrafish (Danio rerio).

Quick Overview for NKX6-1 antibody (N-Term) (ABIN784751)

Target: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Reactivity: Human, Mouse, Rat, Dog, Horse, Pig, Rabbit, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NKX6-1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-NKX6-1 Antibody

Binding Specificity: N-Term

Sequence: MLAVGAMEGT RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPAGPPSS

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against NKX6-1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NKX6-1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 367 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NKX6-1

Target: NKX6-1 (NK6 Homeobox 1 (NKX6-1))

Alternative Name: NKX6-1

Background: NKX6-1 is the transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. NKX6-1 is involved in transcriptional regulation in islet beta cells. Binds to the insulin promoter and is involved in regulation of the insulin gene. Together with NKX2-2 and IRX3, NKX6-1 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. NKX6-1 belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals.In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-300 U66797.1 1-300 301-307 AC096766.3 119394-119400 c 308-676 U66797.1 308-676 677-849 U66798.1 7-179 850-1116 U66799.1 7-273
Alias Symbols: NKX6.1, NKX6A
Protein Size: 367

Molecular Weight: 38 kDa

Gene ID: 4825

NCBI Accession: NM_006168, NP_006159

UniProt: P78426

Pathways: Dopaminergic Neurogenesis, Positive Regulation of Peptide Hormone Secretion