SREBF2 antibody (Middle Region)

Catalog No. ABIN786556

This Rabbit Polyclonal antibody specifically detects SREBF2 in WB and IHC. It exhibits reactivity toward Human, Rat, Dog, Cow, Guinea Pig and Horse.

Quick Overview for SREBF2 antibody (Middle Region) (ABIN786556)

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Reactivity: Human, Rat, Dog, Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SREBF2 Antibody

Binding Specificity: Middle Region

Sequence: VMMGQEKVPI KQVPGGVKQL EPPKEGERRT THNIIEKRYR SSINDKIIEL

Cross-Reactivity: Dog (Canine), Chicken, Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SREBF2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SREBF2

Application Details

Application Notes: WB Suggested Anti-SREBF2 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:7812500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 681 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for SREBF2

Target: SREBF2 (Sterol Regulatory Element Binding Transcription Factor 2 (SREBF2))

Alternative Name: SREBF2 / SREBP2

Background: SREBF2 encodes a ubiquitously expressed transcription factor that controls cholesterol homeostasis by stimulating transcription of sterol-regulated genes. The encoded protein contains a basic helix-loop-helix-leucine zipper (bHLH-Zip) domain.
Alias Symbols: SREBP2, bHLHd2
Protein Interaction Partner: UBC, ERLIN2, MRPS6, NAE1, MAPK3, MAPK1, PARK7, SCAP, SREBF2, SREBF1, NPAT, HINFP, TRRAP, SUMO2, RNF139, SMAD3, HNF4A, ATF6, KLF13, LZTR1, ABLIM2, ATF7IP, ZDHHC17, COPS5, OS9, PIAS3, CASP8AP2, ARHGEF1, PIAS2, SLC4A4, PIAS1, ZYX, ZMYM2, UBE2I, TTC1, SPTBN1,
Protein Size: 681

Molecular Weight: 74 kDa

Gene ID: 6721

NCBI Accession: NM_004599

UniProt: Q12772

Pathways: Regulation of Lipid Metabolism by PPARalpha