Notch 1 (NOTCH1) antibody

Antigen: Notch 1 (NOTCH1)

Synonyms: hN1, TAN1, Mis6, Tan1, lin-12, 9930111A19Rik, NOTCH, NOTCH1, notch1, hn1, tan1, notch, xotch, xnotch, notch-1, xnotch1

Clonality: Monoclonal (MN1A)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Catalog no.: ABIN807923

Additional Information

Alternative name: Notch 1

Gene ID: 4851

Immunogen: GST fusion protein containing cdc10-NCR region of mouse Notch1

Isotype: IgG1

Clone: MN1A

Specificity: The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

Comments:

Transcription Regulation & Nuclear Proteins (Rodent), Transcription Regulation & Nuclear Proteins (Human)
Negative Species: Rat

Application Details

Purification: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8°C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20°C. Avoid freeze/thaw cycles.

Publications

Huppert, Le, Schroeter et al.: "Embryonic lethality in mice homozygous for a processing-deficient allele of Notch1." in: Nature, Vol. 405, Issue 6789, pp. 966-70, 2000 (PubMed).

Espinosa, Santos, Inglés-Esteve et al.: "p65-NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N-CoR." in: Journal of cell science, Vol. 115, Issue Pt 6, pp. 1295-303, 2002 (PubMed). Further details: �

Sun, Li, Vercherat et al.: "Stra13 regulates satellite cell activation by antagonizing Notch signaling." in: The Journal of cell biology, Vol. 177, Issue 4, pp. 647-57, 2007 (PubMed).

Tagami, Okochi, Yanagida et al.: "Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch-1." in: Molecular and cellular biology, Vol. 28, Issue 1, pp. 165-76, 2007 (PubMed).

Watanabe, Nagaoka, Lee et al.: "Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1." in: The Journal of cell biology, Vol. 187, Issue 3, pp. 343-53, 2009 (PubMed).

Kefas, Comeau, Floyd et al.: "The neuronal microRNA miR-326 acts in a feedback loop with notch and has therapeutic potential against brain tumors." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 29, Issue 48, pp. 15161-8, 2009 (PubMed).

Khwaja, Liu, Tong et al.: "HIV-1 Rev-binding protein accelerates cellular uptake of iron to drive Notch-induced T cell leukemogenesis in mice." in: The Journal of clinical investigation, Vol. 120, Issue 7, pp. 2537-48, 2010 (PubMed).