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Notch1 antibody (AA 20-216) (Atto 488)

NOTCH1 Reactivity: Mouse WB, IF, ICC Host: Mouse Monoclonal S253-32 Atto 488
Catalog No. ABIN2483190
  • Target See all Notch1 (NOTCH1) Antibodies
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Binding Specificity
    • 16
    • 16
    • 13
    • 11
    • 7
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 20-216
    Reactivity
    • 115
    • 88
    • 49
    • 9
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 94
    • 38
    • 6
    • 3
    • 2
    • 1
    Mouse
    Clonality
    • 89
    • 51
    • 2
    Monoclonal
    Conjugate
    • 67
    • 10
    • 9
    • 8
    • 6
    • 6
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Notch1 antibody is conjugated to Atto 488
    Application
    • 94
    • 50
    • 48
    • 30
    • 27
    • 26
    • 21
    • 20
    • 15
    • 11
    • 10
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
    Specificity
    Detects >270 kDa. Does not cross-react with Notch 2 or Notch3.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 20-216 (extracellular N-terminus, EGF-like domains 1-5) of mouse Notch1
    Clone
    S253-32
    Isotype
    IgG1
    Top Product
    Discover our top product NOTCH1 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN2483190 was sufficient for detection of Notch1 in 20 μg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Conjugated antibodies should be stored at 4°C
  • Target
    Notch1 (NOTCH1) (Notch 1 (NOTCH1))
    Alternative Name
    Notch1 (NOTCH1 Products)
    Synonyms
    notch1 antibody, NOTCH1 antibody, hn1 antibody, tan1 antibody, notch antibody, xotch antibody, xnotch antibody, notch-1 antibody, xnotch1 antibody, TAN1 antibody, hN1 antibody, 9930111A19Rik antibody, Mis6 antibody, N1 antibody, Tan1 antibody, lin-12 antibody, NOTCH antibody, Xotch antibody, notch1-a antibody, notch 1 antibody, fibropellin-1 antibody, Notch homolog 1, translocation-associated (Drosophila) antibody, Notch homolog 1, translocation-associated antibody, notch 1 S homeolog antibody, notch1 antibody, LOC100631526 antibody, NOTCH1 antibody, Notch1 antibody, notch1.S antibody
    Background
    Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.
    Gene ID
    18128
    NCBI Accession
    NP_032740
    UniProt
    Q01705
    Pathways
    Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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