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AKT 1/2/3 antibody (pTyr312, pTyr315, pTyr316) (Alexa Fluor 350)

Reactivity: Human, Mouse, Rat WB, FACS, IF (cc), IF (p) Host: Rabbit Polyclonal Alexa Fluor 350
Catalog No. ABIN879452
  • Target See all AKT 1/2/3 products
    AKT 1/2/3
    Binding Specificity
    • 24
    • 23
    • 22
    • 22
    • 20
    • 20
    • 18
    • 18
    • 16
    • 16
    • 16
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr312, pTyr315, pTyr316
    Reactivity
    • 164
    • 152
    • 138
    • 46
    • 31
    • 28
    • 25
    • 15
    • 9
    • 6
    • 4
    • 4
    Human, Mouse, Rat
    Host
    • 162
    • 2
    Rabbit
    Clonality
    • 151
    • 13
    Polyclonal
    Conjugate
    • 42
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    This AKT 1/2/3 antibody is conjugated to Alexa Fluor 350
    Application
    • 150
    • 92
    • 91
    • 90
    • 36
    • 33
    • 29
    • 21
    • 9
    • 7
    • 6
    Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    Specificity
    This antibody will detect AKT 1 when phosphorylated at Tyr315 in Human, Mouse, Rat or Bovine, AKT2 when phosphorylated at Tyr316 in Human, Mouse, Rat, or Bovine, and AKT3 when phosphorylated at Tyr312 in Human, Mouse, Rat, or Bovine.
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Dog,Cow,Sheep,Pig,Chicken,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr315
    Isotype
    IgG
  • Application Notes
    FCM 1:20-100
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    AKT 1/2/3
    Alternative Name
    AKT1+2+3 (AKT 1/2/3 Products)
    Background

    Synonyms: AKT1/AKT2/AKT3 Tyr315+Tyr316+Tyr312, AKT1/AKT2/AKT3Tyr315/316/312, AKT1/2/3 phospho Y315 + Y316 + Y312, AKT 1, AKT, AKT1, AKT-1, AKT1_HUMAN, AKT 2, AKT2, AKT-2, AKT2_HUMAN, AKT 3, AKT3, AKT-3, AKT3_HUMAN, C AKT, cAKT, MGC9965, MGC99656, Oncogene AKT1, PKB, PKB alpha, PKB-ALPHA, PKB beta, PKB gamma, PRKBA, Protein Kinase B Alpha, Protein kinase B, Proto-oncogene c-Akt, RAC Alpha, RAC alpha serine/threonine protein kinase, RAC, RAC PK Alpha, Rac protein kinase alpha, RAC Serine/Threonine Protein Kinase, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha, v akt murine thymoma viral oncogene homolog 1, vAKT Murine Thymoma Viral Oncogene Homolog 1.

    Background: AKT, also known as protein kinase B (PKB), is a 57 kDa serine/threonine protein kinase. There are three mammalian isoforms of Akt: AKT1 (PKB alpha), AKT2 (PKB beta) and AKT3 (PKB gamma) with AKT2 and AKT3 being approximately 82 % identical with the AKT1 isoform. Each isoform has a pleckstrin homology (PH)domain, a kinase domain and a carboxy terminal regulatory domain. AKT was originally cloned from the retrovirus AKT8, and is a key regulator of many signal transduction pathways. Its tight control over cell proliferation and cell viability are manifold, overexpression or inappropriate activation of AKT has been seen in many types of cancer. AKT mediates many of the downstream events of phosphatidylinositol 3 kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI3 kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus.

    Gene ID
    207, 208, 10000
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