T7-Tag antibody

Catalog No. ABIN920810

Product Details anti-T7-Tag Antibody

Target: T7-Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This T7-Tag antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Sequence: MASMTGGQQM G

Purification: Affinity purified

Application Details

Application Notes: Western blot: Use at 1:5,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.2 and 0.1% sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for T7-Tag

Target: T7-Tag

Alternative Name: T7 tag (T7-Tag Products)

Target Type: Tag

Background: Rabbits were immunized with MASMTGGQQMG (T7 epitope) conjugated to KLH. After multiple immunizations in Freund's adjuvant, serum was collected. Antibodies were immunoaffinity-purified using the peptide conjugated to a solid-phase support. Specification: The affinity-purified antibodies were analyzed using ELISA and Western blot analyses. The anti-T7 diluted 1:35,000 gave an OD = 1.0 in a 15 minute reaction against peptide conjugated with a different carrier than was used for purification. HRP-conjugated donkey anti-rabbit IgG was used and TMB was the substrate.