ZAP70 antibody

Catalog No. ABIN920874

The Rabbit Polyclonal anti-ZAP70 antibody (ABIN920874) specifically detects ZAP70 in WB. The antibody is reactive with Human samples.

Quick Overview for ZAP70 antibody (ABIN920874)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZAP70 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ZAP70 Antibody

Specificity: Reacts with the C-terminal part of human ZAP- 70 protein tyrosine kinase. The ZAP-70 (zeta- associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP- 70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. ZAP-70 is a cytosolic protein that migrates at 70 kDa in SDS-PAGE. It contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. The crystal structure of the ZAP-70 SH2 domains in complex with a TCR zeta subunit peptide was described. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a co-localization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP- 76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.

Characteristics: Positive Control: Jurkat T cell line. Gene Location: Human chromosome 2q12

Purification: Purified

Immunogen: Bacterially expressed fusion protein representing the C-terminal part (160 amino acids) of human ZAP-70.

Application Details

Application Notes: Western blot: Use at a 1:1,000 dilution. Use standard reducing SDS-sample buffer.

Restrictions: For Research Use only

Handling

Buffer: 100 µg purified pAb at 1 mg/mL from rabbit antiserum in PBS with 15 mM sodium azide, pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Alternative Name: ZAP-70

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway