Caspase 1 antibody (Middle Region)

Catalog No. ABIN929105

The Rabbit Polyclonal anti-Caspase 1 antibody is suitable to detect Caspase 1 in samples from Human. It has been validated for WB.

Quick Overview for Caspase 1 antibody (Middle Region) (ABIN929105)

Target: Caspase 1 (CASP1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Caspase 1 Antibody

Binding Specificity: Middle Region

Purification: Purified

Immunogen: CASP1 antibody was raised in rabbit using the middle region of CASP1 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

CASP1 Blocking Peptide, ABIN5612630, is also available for use as a blocking control in assays to test for specificity of this CASP1 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for Caspase 1

Target: Caspase 1 (CASP1)

Alternative Name: CASP1

Background: This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. Synonyms: Polyclonal CASP1 antibody, Anti-CASP1 antibody, caspase 1, apoptosis-related cysteine peptidase, interleukin 1, beta, convertase antibody, ICE antibody, IL1BC antibody, P45 antibody.

Pathways: Apoptosis, Interferon-gamma Pathway, Positive Regulation of Endopeptidase Activity, Inflammasome