ATG5 antibody (Middle Region)

Catalog No. ABIN929121

The Rabbit Polyclonal anti-ATG5 antibody (ABIN929121) specifically detects ATG5 in WB. The antibody is reactive with Human samples.

Quick Overview for ATG5 antibody (Middle Region) (ABIN929121)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATG5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ATG5 Antibody

Binding Specificity: Middle Region

Purification: Purified

Immunogen: ATG5 antibody was raised in rabbit using the middle region of ATG5 as the immunogen

Application Details

Application Notes: WB: 0.2-1 µg/mL
Optimal conditions should be determined by the investigator.

Comment:

ATG5 Blocking Peptide, (ABIN5612210), is also available for use as a blocking control in assays to test for specificity of this ATG5 antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized powder. Add 50 µL of distilled water. Final antibody concentration is 1 mg/mL in PBS buffer.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C, following reconstitution, aliquot and store at -20 °C.

Target Details for ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Alternative Name: ATG5

Background: ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity. Synonyms: Polyclonal ATG5 antibody, Anti-ATG5 antibody, ATG5 autophagy related 5 homolog, S. cerevisiae antibody, APG5 antibody, APG5-LIKE antibody, APG5L antibody, ASP antibody, hAPG5 antibody.

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy