p300 antibody

Catalog No. ABIN931392

The Mouse Monoclonal anti-p300 antibody (Clone 7D8A6) (ABIN931392) specifically detects p300 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for p300 antibody (ABIN931392)

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This p300 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 7D8A6

Product Details anti-p300 Antibody

Immunogen: EP300 antibody was raised in Mouse using a purified recombinant fragment of EP300 expressed in E. coli as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Target Details for p300

Target: p300 (EP300) (E1A Binding Protein P300 (EP300))

Alternative Name: EP300

Background: EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CBP (CREB-binding protein), and like CBP can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Synonyms: Monoclonal EP300 antibody, Anti-EP300 antibody, E1A binding protein p300 antibody, p300 antibody.

Pathways: p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size