Lhca3 antibody

Catalog No. ABIN93513

Product Details anti-Lhca3 Antibody

Target: Lhca3 (psi Type III Chlorophyll A/B-Binding Protein (Lhca3))

Reactivity: Spinach, Zea mays, Arabidopsis thaliana, Barley, Green bean, Oryza sativa, Pisum sativum

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Cross-Reactivity (Details): peptide target used to elicit this antibody is not conserved in type II Rubisco foundin dinoflagellates and some photosynthetic bacteria. For those species product.

Characteristics: Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana

Purification: affinity purified

Immunogen: BSA-conjugated synthetic peptide derived from the Lhca3 protein sequence from Arabidopsis thaliana UniProt: Q9SY97, TAIR: At1g61520. This sequence is highly conserved in Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms.

Isotype: IgG

Application Details

Application Notes: Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 µL of sterile water

Buffer: PBS pH 7.4

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

References for anti-Lhca3 antibody (ABIN93513)

Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

Yadavalli, Neelam, Rao, Reddy, Subramanyam: "Differential degradation of photosystem I subunits under iron deficiency in rice." in: Journal of plant physiology, Vol. 169, Issue 8, pp. 753-9, (2012) (PubMed).

Target Details for Lhca3

Target: Lhca3 (psi Type III Chlorophyll A/B-Binding Protein (Lhca3))

Synonyms: LHCA3*1 antibody, LHCI-680 antibody, T25B24.12 antibody, T25B24_12 antibody, photosystem I light harvesting complex gene 3 antibody, PSI type III chlorophyll a/b-binding protein antibody, LHCA3 antibody

Background: AGI Code: At1g61520
The light-harvesting protein Lhca3 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca3 is imported as a precursor from the cytosol into the chloroplast. Upon integration in the thylakoid membrane Lhca3 forms a heterodimer (LHCI-680) with Lhca2 that associates with the PSI core close to PsaF and PsaK.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073.

Molecular Weight: expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana

UniProt: Q9SY97