Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody

Antigen: Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1)

Synonyms: B1, S7, Bp35, CD20, MS4A2, LEU-16, MGC3969, Cd20, Ly-44, Ms4a2, AA960661, FcRB, Fce1b, Ms4a1, Fcer1b, Fcrbeta, FCIGA, MS4A1, bp35, cd20, ms4a2, leu-16, ms4a4c, cd20-like

Clonality: Monoclonal (LT20)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN94027

Additional Information

Alternative name: CD20

Immunogen: Normal human lymphocytes from lymph node.

Format: Purified

Isotype: IgG2a  (Matching secondary antibodies)

Clone: LT20

Description: CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDanon-glycosylated surface phosphoprotein expressed on mature and most malignant B cells,but not stem cells or plasma cells (low number of the CD20 has been also detected on asubpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Itsexpression on B cells is synchronous with the expression of surface IgM. CD20 regulatestransmembrane calcium conductance (probably functioning as a component ofstore-operated calcium channel), cell cycle progression and B-cell proliferation. It isassociated with lipid rafts, but the intensity of this association depends on extracellulartriggering, employing CD20 conformational change and/or BCR (B cell antigen receptor)aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidlydissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it isexpressed at high levels on most B-cell malignancies, but does not become internalized orshed from the plasma membrane following mAb treatment.

Specificity: The antibody LT20 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membranereceptor with four transmembrane domains, expressed on B lymphocytes (it is lost onplasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods and ion exchange chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Flow Cytometry analysis

Publications

Publications: Cupedo, Crellin, Papazian et al.: "Human fetal lymphoid tissue-inducer cells are interleukin 17-producing precursors to RORC+ CD127+ natural killer-like cells." in: Nature immunology, Vol. 10, Issue 1, pp. 66-74, 2008 (PubMed).