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CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (Dimer gamma-epsilon) (PE)

Details for Product anti-CD3E Antibody No. ABIN94062, Supplier: Log in to see
Antigen
  • cd3e
  • CD3E
  • t3e
  • tcre
  • AI504783
  • CD3
  • CD3epsilon
  • T3e
  • T3E
  • TCRE
Alternatives
anti-Human CD3E antibody for Flow Cytometry
Epitope
Dimer gamma-epsilon, Dimer delta-epsilon
31
15
14
13
13
10
8
8
5
5
4
4
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
392
242
61
45
7
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Host
Mouse
204
160
155
77
33
21
1
Clonality (Clone)
Monoclonal ()
Conjugate
This CD3E antibody is conjugated to PE
87
66
60
40
17
15
11
10
7
7
5
5
5
5
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS)
459
176
174
133
67
63
51
46
34
24
20
11
9
6
4
3
3
2
2
1
1
1
1
1
1
1
1
Supplier
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Immunogen Human thymocytes and T lymphocytes.
Clone MEM-57
Isotype IgG2a
Specificity The antibody MEM-57 reacts with gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multisubunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes. HLDA IV., WS Code T 96
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Alternative Name CD3E (CD3E Antibody Abstract)
Background CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex.T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-82 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells
Pathways TCR Signaling
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µL reagent / 100 µL of whole blood or 10^6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Supplier Images
Flow Cytometry (FACS) image for anti-CD3E antibody (CD3e Molecule, epsilon (CD3-TCR Complex)) (Dimer gamma-epsilon) (PE) (ABIN94062) Surface staining of human peripheral blood cells with anti-human CD3 (MEM-57) FITC. C...
Product cited in: Majer, Vlaskova, Krol et al.: "Danon disease: a focus on processing of the novel LAMP2 mutation and comments on the beneficial use of peripheral white blood cells in the diagnosis of LAMP2 deficiency." in: Gene, Vol. 498, Issue 2, pp. 183-95, 2012 (PubMed).

Cancer: Lesport, Baudhuin, Sousa et al.: "Inhibition of human Vγ9Vδ2 T-cell antitumoral activity through HLA-G: implications for immunotherapy of cancer." in: Cellular and molecular life sciences : CMLS, 2011 (PubMed). (Sample species: Human).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Panyi, Bagdány, Bodnár et al.: "Colocalization and nonrandom distribution of Kv1.3 potassium channels and CD3 molecules in the plasma membrane of human T lymphocytes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2592-7, 2003 (PubMed).

Dave, Cao, Browne et al.: "CD3 delta deficiency arrests development of the alpha beta but not the gamma delta T cell lineage." in: The EMBO journal, Vol. 16, Issue 6, pp. 1360-70, 1997 (PubMed).

Hilgert, Fran?k, Stefanová et al.: "Therapeutic in vivo use of the A1-CD3 monoclonal antibody." in: Transplantation, Vol. 55, Issue 2, pp. 435-8, 1993 (PubMed).

Soucek, Hilgert, Budová et al.: "Augmentation of NK cell activity and proliferation in cultured lymphocytes of leukemic patients by monoclonal antibodies CD3 and interleukin-2." in: Neoplasma, Vol. 41, Issue 2, pp. 75-81, 1994 (PubMed).

Soucek, Chudomel, Hrubá et al.: "Induction of NK and LAK activities in human lymphocyte culture by a cytosol fraction from leukemic myeloblasts and by monoclonal antibody CD 3." in: Neoplasma, Vol. 38, Issue 1, pp. 33-41, 1991 (PubMed).

Background publications Muhammad, Schiller, Forster et al.: "Sequential cooperation of CD2 and CD48 in the buildup of the early TCR signalosome." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 12, pp. 7672-80, 2009 (PubMed).

Todaro, DAsaro, Caccamo et al.: "Efficient killing of human colon cancer stem cells by gammadelta T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 11, pp. 7287-96, 2009 (PubMed).

Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Jursik, Prchal, Grillari-Voglauer et al.: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009 (PubMed).

Paster, Paar, Eckerstorfer et al.: "Genetically encoded Förster resonance energy transfer sensors for the conformation of the Src family kinase Lck." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 182, Issue 4, pp. 2160-7, 2009 (PubMed).

Houben, Voigt, Noelke et al.: "MAPK-independent impairment of T-cell responses by the multikinase inhibitor sorafenib." in: Molecular cancer therapeutics, Vol. 8, Issue 2, pp. 433-40, 2009 (PubMed).

Alarcón, Swamy, van Santen et al.: "T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity." in: EMBO reports, Vol. 7, Issue 5, pp. 490-5, 2006 (PubMed).

Kuhns, Davis, Garcia: "Deconstructing the form and function of the TCR/CD3 complex." in: Immunity, Vol. 24, Issue 2, pp. 133-9, 2006 (PubMed).

Huang, Wange: "T cell receptor signaling: beyond complex complexes." in: The Journal of biological chemistry, Vol. 279, Issue 28, pp. 28827-30, 2004 (PubMed).

Horejsí, Angelisová, Bazil et al.: "Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) ..." in: Folia biologica, Vol. 34, Issue 1, pp. 23-34, 1988 (PubMed).