CD4 Molecule (CD4) antibody (Biotin)

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (MEM-241)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Catalog no.: ABIN94089

Additional Information

Alternative name: CD4

Gene ID: 12pter-p12

Immunogen: 2 N-terminal domains of human CD4 fused to human IgG1 Fc

Isotype: IgG1

Clone: MEM-241

Description: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulinsupergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-likeV-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consistsof 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain ofCD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4segregates into specific detergent-resistant T-cell membrane microdomains.Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1),HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds toCD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1),L-selectinIntracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHCclass II molecules) and HIV infection (human immunodeficiency virus, CD4 is primaryreceptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-celladhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigenpresentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost completeabsence in patients blood, tissue and organs (SCID immunodeficiency).

Specificity: The antibody MEM-241 recognizes CD4 antigen, a 55 kDa transmebrane glycoproteinexpressed on a subset of T lymphocytes (helper T-cells) and also on monocytes, tissuemacrophages and granulocytes.HCDM (former HLDA VIII) Meeting, May 2006, Québec, Canada, WS Code M241

Application Details

Application Notes: Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 1500.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Reactivity of the antibody MEM-241 with human CD4 immunoprecipitated from T cells by the antibody MEM-115. T cells isolated from fresh buffy coats were solubilized in lauryl maltoside lysis buffer and the lysate was subjected to affinity chromatography both on an immunosorbent made of immobilized antibody MEM-115 (CD4) or irrelevant negative control immunosorbent. Western blot was stained with the antibody MEM-241. Lanes: 1. original lysate, 2. the material eluted from irrelevant immunosorbent (negative control), 3. the material eluted from MEM-115 (CD4)-Sepharose.

Publications

Publications: Manasa, Musabaike, Masimirembwa et al.: "Evaluation of the Partec flow cytometer against the BD FACSCalibur system for monitoring immune responses of human immunodeficiency virus-infected patients in Zimbabwe." in: Clinical and vaccine immunology : CVI, Vol. 14, Issue 3, pp. 293-8, 2007 (PubMed).

Anderson, Sayers, Haniffa et al.: "Differential regulation of naïve and memory CD4+ T cells by alternatively activated dendritic cells." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 124-33, 2008 (PubMed).

Karlsson, Cowley, Martinez et al.: "Homogeneous monocytes and macrophages from human embryonic stem cells following coculture-free differentiation in M-CSF and IL-3." in: Experimental hematology, Vol. 36, Issue 9, pp. 1167-75, 2008 (PubMed).