CD4 Molecule (CD4) antibody

Antigen: CD4 Molecule (CD4)

Synonyms: CD4, MGC165891, cd4, CD4mut, L3T4, Ly-4, p55, W3/25

Clonality: Monoclonal (MEM-16)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Catalog no.: ABIN94103

Additional Information

Alternative name: CD4

Gene ID: 12pter-p12

Immunogen: HPB cell line (human peripheral blood leukemia T-cells)

Format: Purified

Isotype: IgM  (Matching secondary antibodies)

Clone: MEM-16

Description: CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulinsupergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-likeV-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consistsof 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain ofCD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4segregates into specific detergent-resistant T-cell membrane microdomains.Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1),HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds toCD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1),L-selectinIntracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHCclass II molecules) and HIV infection (human immunodeficiency virus, CD4 is primaryreceptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-celladhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigenpresentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost completeabsence in patients blood, tissue and organs (SCID immunodeficiency).

Specificity: The antibody MEM-16 recognizes an epitope EF loop of D1 domain of CD4 antigen, a 55kDa transmebrane glycoprotein expressed on a subset of T lymphocytes (helper T-cells)and also on monocytes, tissue macrophages and granulocytes.HLDA V, WS Code T T-CD04.10HLDA VI, WS Code T 6T-013 This antibody does not react with: Porcine.

Application Details

Application Notes: Flow Cytometry: Recommended dilution: 10 µg/ml. Positive control: Peripheral blood lymphocytes

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by precipitation methods and size-exclusion chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. Do not freeze.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).