Intercellular Adhesion Molecule 1 (ICAM1) antibody (Biotin)

Antigen: Intercellular Adhesion Molecule 1 (ICAM1)

Synonyms: BB2, CD54, P3.58, Ly-47, Icam-1, MALA-2, MGC6195, ICAM-1, ICAM1, ICAM

Clonality: Monoclonal (1H4)

Host: Mouse

Reactivity: Human

Conjugate: Biotin

Catalog no.: ABIN94179

Additional Information

Alternative name: CD54

Immunogen: Raji cells and spleen cells fused with NS1 cells

Isotype: IgG2b

Clone: 1H4

Description: CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is atransmembrane molecule with 7 potential N-glycosylated sites, expressed on restingmonocytes and endothelial cells and can be upregulated on many other cells, e.g. withlymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also onkeratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion bybinding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction ofCD54 with LFA-1 enhances antigen-specific T-cell activation.

Specificity: The antibody 1H4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembraneglycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, Tlymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells,the expression of CD54 is upregulated by activation.

Application Details

Application Notes: Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 200.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 1 mg/ml

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: CD Antigens, Surface Receptors of Immune Cells, Immunology, Inflammation, Infection

Restrictions: For Research Use only

Images

Flow Cytometry analysis