PAG / CBP antibody

Antigen: PAG / CBP

Clonality: Monoclonal (PAG-C1)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus), Cow (Bovine)

Application: Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN94270

Additional Information

Immunogen: C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH.

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: PAG-C1

Description: PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is aubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts(glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gelsanomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src familykinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the majornegative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells orhigh affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosinephosphorylation and Csk binding, while T cell receptor signaling causes PAGdephosphorylation, loss of Csk binding and increased activation of the protein tyrosinekinase Lck.

Specificity: The antibody PAG-C1 recognizes an epitope located in the C-terminal domain ofCsk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptorprotein present in membrane rafts (glycosphingolipid-enriched microdomains), whichhowever migrates on SDS PAGE gels anomalously as an 80 kDa molecule.

Application Details

Application Notes: Immunoprecipitation - Positive control: RAJI human Burkitt lymphoma cell line. Immunohistochemistry (paraffin sections) - Positive tissue: Appendix (germinal center of lymphatic follicle. Application note: heat-mediated antigen retrieval in citrate buffer pH 6. 1

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunoprecipitation of human PAG/Cbp from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human PAG (MEM-255). Note: PAG/Cbp is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-PAG (PAG-C1). Lane 3: immunoprecipitation with anti-PAG (polyclonal antibody)

Immunohistochemistry staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells

Publications

Publications: Baumgartner, Angelisovua, Setterblad et al.: "Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes." in: Blood, Vol. 101, Issue 5, pp. 1874-81, 2003 (PubMed).

Davidson, Bakinowski, Thomas et al.: "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." in: Molecular and cellular biology, Vol. 23, Issue 6, pp. 2017-28, 2003 (PubMed).

Vang, Abrahamsen, Myklebust et al.: "Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling." in: The Journal of biological chemistry, Vol. 278, Issue 20, pp. 17597-600, 2003 (PubMed).