Cytokeratin 18 (KRT18) antibody

Antigen: Cytokeratin 18 (KRT18)

Synonyms: K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18

Clonality: Monoclonal (DC-10)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Immunocytochemistry (ICC)

Catalog no.: ABIN94282

Additional Information

Alternative name: Cytokeratin 18

Gene ID: 17q24.2

Immunogen: Human breast carcinoma cell line PMC-42.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: DC-10

Description: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins).

Specificity: The antibody DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member ofintermediate filaments subfamily represented in epithelial tissues. This antibody does not react with: Porcine, Mouse, Rat, Hamster, Bovine, Sheep, Dog (Canine).

Application Details

Application Notes: Western Blotting - Recommended dilution: 1-2 µg/ml, overnight in 4 o C Positive control: HeLa human cervix carcinoma cell lineMCF-7 human breast adenocarcinoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel). Immunohistochemistry (paraffin sections) - Recommended dilution: 1: 10 - 1: 100Positive tissue: epithelial tissue (skin) -. Immunocytochemistry: Positive control: MCF-7 human breast adenocarcinoma cell lineHeLa human cervix carcinoma cell lineELISA - Positive control: MCF-7 human breast adenocarcinoma cell line. Application note: The antibody DC-10 has been tested as the detection antibody in asandwich ELISA for analysis of Cytokeratin 18 in combination with the antibody C-04 (cat. no. 11-106-C100).

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Signaling, Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use only

Images

Detection of cytokeratin 18 in HeLa cell lysate by mouse monoclonal antibody DC-10. 

Publications

Publications: Ta: "Primary human hepatocytes on biodegradable poly(l-lactic acid) matrices: a promising model for improving transplantation efficiency with tissue engineering." in: Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society, Vol. 17, Issue 2, pp. 104-14, 2011 (PubMed).