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Antibodies
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anti-Cytokeratin 18 (KRT18) antibody (Biotin)
Cytokeratin 18 (KRT18) antibody (Biotin)
| Antigen |
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| Synonyms |
K18, CK18, Krt1-18, CYK18, KRT18, LOC100008885, MGC109143, krt18 |
| Clonality |
Monoclonal (DC-10) |
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Host
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Reactivity
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Human (141), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (10), Hamster (5), None (4), Sheep (Ovine) (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1)
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Conjugate
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FITC (13), Biotin (10), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Application
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Western Blotting (WB) (84), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (77), Immunofluorescence (IF) (67), Flow Cytometry (FACS) (66), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (22), ELISA (20), Immunoprecipitation (IP) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2)
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| Catalog no. |
ABIN94283 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
325.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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Cytokeratin 18
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Gene ID
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17q24.2
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Immunogen
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Human breast carcinoma cell line PMC-42.
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Isotype
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IgG1
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Clone
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DC-10
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Description
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Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratin 18 belongs to type I family (acidic cytokeratins).
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Specificity
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The antibody DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member ofintermediate filaments subfamily represented in epithelial tissues. This antibody does not react with: Porcine, Mouse, Rat, Hamster, Bovine, Sheep, Dog (Canine) .
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Application Notes
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The reagent is designed for Flow cytometry. Suggested working dilution is 1: 500.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
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Concentration
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1 mg/ml
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Preparation: The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. Thereagent is free of unconjugated biotin..
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Signaling, Cytoskeleton, Extracellular Matrix
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Restrictions
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For Research Use only
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Alternatives for antigen "Cytokeratin 18 (KRT18)", type "Antibodies"
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Hosts
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Mouse (88), Rabbit (78), Guinea Pig (1), Sheep (1)
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Reactivities
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Human (141), Rat (Rattus) (57), Mouse (Murine) (56), Dog (Canine) (41), Rabbit (35), Cow (Bovine) (23), Pig (Porcine) (22), Chicken (20), Horse (Equine) (17), Mammalian (10), Hamster (5), None (4), Sheep (Ovine) (3), Bird (Avian) (2), Cat (Feline) (2), Monkey (1)
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Applications
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Western Blotting (WB) (84), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (77), Immunofluorescence (IF) (67), Flow Cytometry (FACS) (66), Immunohistochemistry (IHC) (33), Immunohistochemistry (Frozen Sections) (IHC (fro)) (28), Enzyme Immunoassay (EIA) (22), ELISA (20), Immunoprecipitation (IP) (18), Immunocytochemistry (ICC) (12), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), ELISA (Detection) (2), Immunoelectron Microscopy (IEM) (2)
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Conjugates
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FITC (13), Biotin (10), Alexa Fluor 488 (3), HRP (3), Alexa Flour 488 (2), Alexa Fluor 350 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2), RBITC (2), Alexa Fluor 700 (1)
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Epitopes
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pSer33 (7), C-Term (5), Center (1), N-Term (1)
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Alternatives 
