Cytokeratin 8 (KRT8) antibody

Antigen: Cytokeratin 8 (KRT8)

Synonyms: K8, KO, CK8, CYK8, K2C8, CARD2, K18, CYK18

Clonality: Monoclonal (C-43)

Host: Mouse

Reactivity: Human, Pig (Porcine), Cow (Bovine), Sheep (Ovine), Rabbit

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN94300

Additional Information

Alternative name: Cytokeratin 8

Immunogen: Cytoskeleton preparation from HeLa human cervix carcinoma cell line.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: C-43

Description: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkablebiochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20different polypeptides, molecular weight between 40 kDa and 68 kDa. The individualcytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidiccytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Specificity: The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa). Cytokeratins are a member ofintermediate filaments subfamily represented in epithelial tissues. This antibody does not react with: Mouse, Rat, Hamster, Chicken, Xenopus.

Application Details

Application Notes: Immunohistochemistry (paraffin sections) - Recommended dilution: 20 µg/ml. Positive tissue: prostate. Immunocytochemistry -

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer, Cytoskeleton, Cytokeratins, Extracellular Matrix

Restrictions: For Research Use only

Publications

Publications: Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).