FYN Oncogene Related To SRC, FGR, YES (FYN) antibody

Antigen: FYN Oncogene Related To SRC, FGR, YES (FYN)

Synonyms: FYN, fyn, MGC52878, MGC132140, zgc:109996, c-fyn, zgc:86720, SLK, SYN, MGC45350, AI448320, AW552119, MGC115870, PKC

Clonality: Monoclonal (FYN-01)

Host: Mouse

Reactivity: Human, Mouse (Murine)

Application: Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN94311

Additional Information

Alternative name: Fyn

Immunogen: Bacterially expressed recombinant fragment of human Fyn (aa 7-176).

Format: Purified

Isotype: IgG2b  (Matching secondary antibodies)

Clone: FYN-01

Description: Fyn is a ubiquitously expressed Src-family protein tyrosine kinase with important roles e.g.in immune and nervous system. It regulates N-methyl-D-aspartate (NMDA) receptorfunctions, thus affecting various brain functions, and even many of its other substrates areimportant for neural migration, synaptic plasticity, oligodendrocyte differentiation, and axongrowth and guidance. In immune system Fyn namely regulates the commitment of T cells toactivation, is important in T cell anergy induction, promotes mast cell chemotaxis andreorganization of cytoskeleton and participates in mast cell activation. Fyn is also involved inembryonic stem cell growth and differentiation, associates with tubulin and may play roles inmitotic spindle formation.

Specificity: The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosinekinase.

Application Details

Application Notes: Immunoprecipitation - Positive control: J77 T cell line,Preparation of cell lysate: 30 min on ice (orbital incubator) in lysing buffer, lysing buffer withN-dodecyl beta-D-maltoside (20 mM Tris/Cl, 100 mM NaCl pH 8,2, 1% laurylmaltosid (w/v),50 mM NaF). Application note: The antibody FYN-01 apparently gives very specific signal (by 59 kDa),and nicely immunoprecipitates Fyn from cell lysates. - Recommended dilution: 2 µg/ml. Positive control: JURKAT human T cell leukemia cell line. Immunohistochemistry (paraffin sections) - Recommended dilution: 5 µg/ml. Positive tissue: brainImmunocytochemistry

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Cancer, Tyrosine Kinases, Alzheimer's Disease, Signaling

Restrictions: For Research Use only

Images

Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01). Lane 1: original lysate of T cellsLane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.

Publications

Publications: Queval, Nicolas, Beau: "Role of Src kinases in mobilization of glycosylphosphatidylinositol-anchored decay-accelerating factor by Dr fimbria-positive adhering bacteria." in: Infection and immunity, Vol. 79, Issue 7, pp. 2519-34, 2011 (PubMed).