HLA Class I antibody (Biotin)
| Antigen |
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| Clonality |
Monoclonal (W6-32) |
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Host
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Reactivity
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Conjugate
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| Catalog no. |
ABIN94333 |
| Quantity |
0.1 mg (1 mg/ml) |
| Price |
429.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Alternative name
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HLA-Class I
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Immunogen
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Membrane of human tonsil cells
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Isotype
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IgG2a
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Clone
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W6-32
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Description
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HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteinsexpressed on nucleated cells and noncovalently associated with an invariant beta2microglobulin. They carry foreign determinants important for immune recognition bycytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class Iantigens are represented by HLA-A, HLA-B and HLA-C molecules.
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Specificity
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The antibody W6/32 recognises MHC Class I molecules (MHC Class Ia) that are expressedon the surface of all human nucleated cell types.The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expressionin different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathyand other neuromuscular disorders.This antibody W6/32 is also suitable as a positive control for HLA tissue typing andcrossmatching. This antibody does not react with: Rabbit .
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Application Notes
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Biotinylated antibody is designed for indirect immunofluorescence analysis by FlowCytometry. Suggested working dilution is 1: 300.Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
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Concentration
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1 mg/ml
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Buffer
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Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 Preparation: The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. Thereagent is free of unconjugated biotin..
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Storage
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Research Area
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Major Histocompatibility Complex (MHC)
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Restrictions
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For Research Use only
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Alternatives for antigen "HLA Class I", type "Antibodies"
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Hosts
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Mouse (11)
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Reactivities
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Human (11), Cow (Bovine) (6), Primates (6), Cat (Feline) (4)
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Applications
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Flow Cytometry (FACS) (10), Immunoprecipitation (IP) (4), ELISA (3), Western Blotting (WB) (3), Functional Studies (Func) (2), Immunohistochemistry (Frozen Sections) (IHC (fro)) (2), Immunocytochemistry (ICC) (1)
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Conjugates
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PE (3), Alexa Fluor 488 (1), FITC (1)
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Alternatives 
