Mouse anti-Human IgE Antibody
-
- Target See all IgE products
- IgE
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Application
- ELISA, Flow Cytometry (FACS)
- Specificity
- The antibody BE5 reacts with human IgE, it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.
- Cross-Reactivity (Details)
- Human
- Purification
- Purified by protein-A affinity chromatography.
- Purity
- > 95 % (by SDS-PAGE)
- Immunogen
- Purified human IgE.
- Clone
- BE5
- Isotype
- IgG1
-
-
- Application Notes
- Flow cytometry: Recommended dilution: 5 μg/mL.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Do not freeze.
- Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Do not freeze.
-
- Target
- IgE
- Abstract
- IgE Products
- Background
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
- Molecular Weight
- 180 kDa
-