Lymphocyte-Specific Protein tyrosine Kinase (LCK) antibody

Antigen: Lymphocyte-Specific Protein tyrosine Kinase (LCK)

Synonyms: YT16, p56lck, pp58lck, Lck1, Lcktkr, MGC136695, MGC194004, MGC194043, zgc:136695, LCK, MGC126900

Clonality: Monoclonal (LCK-01)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN94422

Additional Information

Alternative name: Lck

Immunogen: Peptide corresponding to amino acids 22-36 in the sequence of human Lck.

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: LCK-01

Description: Lck is a Src-family tyrosine kinase, which is essential for signaling through the T-cellreceptor (TCR) complex. Upon TCR triggering, Lck phosphorylates the ITAM motives in itszeta subunits, establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70,which is also phosphorylated by Lck and thereby activated to generate subsequent signalingplatforms by phosphorylation of adaptor LAT. Whereas the majority of Lck is localized to theplasma membrane, there is also a significant fraction associated with the Golgi apparatus,which may contribute to Raf activation under conditions of weak stimulation through theTCR. Lck is also involved in the regulation of apoptosis induced by various stimuli, but notby the death receptors.

Specificity: The antibody LCK-01 recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-familyprotein tyrosine kinase. This antibody does not react with: Mouse.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Immunology, Tyrosine Kinases, Adaptive Immunity, CD Antigens, Signaling

Restrictions: For Research Use only

Images

Western Blotting

Publications

Publications: Cebecauer, Cernuy, Horejsui: "Incorporation of leucocyte GPI-anchored proteins and protein tyrosine kinases into lipid-rich membrane domains of COS-7 cells." in: Biochemical and biophysical research communications, Vol. 243, Issue 3, pp. 706-10, 1998 (PubMed).

Romagnoli, Strahan, Pelosi et al.: "A potential role for protein tyrosine kinase p56(lck) in rheumatoid arthritis synovial fluid T lymphocyte hyporesponsiveness." in: International immunology, Vol. 13, Issue 3, pp. 305-12, 2001 (PubMed).

Brdicková, Brdicka, Angelisová et al.: "LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling." in: The Journal of experimental medicine, Vol. 198, Issue 10, pp. 1453-62, 2003 (PubMed).

Ota: "The effects of membrane compartmentalization of Csk on TCR signaling." in: Biochimica et biophysica acta, 2010 (PubMed).

Hrdinka, Dráber, Stepánek et al.: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Stepanek, Kalina, Draber et al.: "Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148." in: The Journal of biological chemistry, Vol. 286, Issue 25, pp. 22101-12, 2011 (PubMed).