LAT2 antibody (Cytoplasmic Domain) (PE)

Catalog No. ABIN94440

Cited in 3+ publications. The PE-conjugated Mouse Monoclonal anti-LAT2 antibody (Clone NAP-07) (ABIN94440) specifically detects LAT2 in ICFC. The antibody is reactive with Human and Mouse samples.

Quick Overview for LAT2 antibody (Cytoplasmic Domain) (PE) (ABIN94440)

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAT2 antibody is conjugated to PE

Application: Intracellular Flow Cytometry (ICFC)

Clone: NAP-07

Product Details anti-LAT2 Antibody (PE)

Binding Specificity: AA 91-243, Cytoplasmic Domain

Purpose: Anti-NTAL PE

Cross-Reactivity (Details): Mouse, Human, Other not tested

Characteristics: The antibody NAP-07 reacts with an intracellular epitope of Non-T cell activation linker (NTAL), also known as LAB (linker of activated B cells), a 25 - 30 kDa transmembrane adaptor protein associating with lipid raft components in the plasma membrane of B lymphocytes, NK cells and myeloid cells.

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Intracellular staining, recommended dilution: 1-5 μg/mL, positive tissue: RAJI human lymphoma cell line, RAMOS human lymphoma cell line.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze,Protect from prolonged exposure to light

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-LAT2 antibody (PE) (ABIN94440)

Tkaczyk, Horejsi, Iwaki, Draber, Samelson, Satterthwaite, Nahm, Metcalfe, Gilfillan: "NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation." in: Blood, Vol. 104, Issue 1, pp. 207-14, (2004) (PubMed).

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, (2004) (PubMed).

Brdicka, Imrich, Angelisová, Brdicková, Horváth, Spicka, Hilgert, Lusková, Dráber, Novák, Engels, Wienands, Simeoni, Osterreicher, Aguado, Malissen, Schraven, Horejsí: "Non-T cell activation linker (NTAL): a transmembrane adaptor protein involved in immunoreceptor signaling." in: The Journal of experimental medicine, Vol. 196, Issue 12, pp. 1617-26, (2002) (PubMed).

Target Details for LAT2

Target: LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))

Alternative Name: NTAL

Background: Linker for activation of T cells family member 2,NTAL (non-T cell activation linker), also known as LAB (linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.,LAB, LAT2

Gene ID: 56743

UniProt: Q9JHL0

Pathways: Fc-epsilon Receptor Signaling Pathway, BCR Signaling