Transferrin antibody (Tf) (N-Term)

Details for Product anti-Tf Antibody No. ABIN94486, Supplier: Log in to see
Antigen
  • ltf
  • pro1557
  • pro2086
  • mgc107777
  • LOC692564
  • LOC100144362
  • tf
  • tf-b
  • MGC64306
  • cb285
  • gavi
  • id:ibd3238
  • id:ibd3525
  • sb:cb285
  • wu:fb57g06
  • wu:fb62h02
  • wu:fb63h10
  • wu:fb64h10
  • zgc:112154
  • 143958_at
  • CG6186
  • Dmel\\CG6186
  • TSF1
  • Tf
  • Trf
  • anon-EST:Posey265
  • tsf1
  • TF
  • PRO1557
  • PRO2086
  • TFQTL1
  • AI266983
  • Cd176
  • HP
  • Tfn
  • hpx
  • LTF
  • TFEW
  • conalbumin
  • transferrin
  • serotransferrin B
  • transferrin-a
  • Transferrin 1
  • liver transferrin
  • transferrin (ovotransferrin)
  • tf
  • LOC692564
  • LOC100144362
  • tf-b
  • AaeL_AAEL011949
  • tfa
  • Tsf1
  • TF
  • TRF
  • Trf
  • Tf
Alternatives
anti-Rabbit Transferrin antibody for Blocking Peptide
Epitope
N-Term
59
9
8
7
6
5
5
5
5
5
5
2
2
1
1
1
1
1
1
1
Reactivity
Human, Pig (Porcine), Rabbit
464
104
79
65
39
19
13
13
12
10
9
6
5
4
4
2
2
1
1
Host
Mouse
309
277
73
27
9
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Transferrin antibody is un-conjugated
55
54
38
10
8
8
7
6
6
6
6
6
6
6
6
6
5
5
5
2
2
2
2
2
2
2
2
2
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
436
300
158
107
83
83
63
53
34
30
26
26
24
22
18
16
11
9
8
8
8
8
6
6
3
3
2
2
2
2
1
1
1
1
1
Options
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Available images

Immunogen Purified porcine transferrin.
Clone HTF-14
Isotype IgG1
Specificity The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
No Cross-Reactivity Cow (Bovine), Sheep (Ovine), Dog (Canine), Horse (Equine)
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name Transferrin (Tf Antibody Abstract)
Background Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 μmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH . Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Research Area Cardiovascular, Serum/Plasma Proteins, Enzymes, Metabolism
Pathways Transition Metal Ion Homeostasis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for anti-Transferrin antibody (Tf) (N-Term) (ABIN94486) Human transferrin detected by the mouse monoclonal antibody HTF-14. 1. hTransferrin; ...
Background publications Steere, Roberts, Byrne, Dennis Chasteen, Bobst, Kaltashov, Smith, Macgillivray, Mason: "Properties of a homogeneous C-lobe prepared by introduction of a TEV cleavage site between the lobes of human transferrin." in: Protein expression and purification, 2010 (PubMed).

Graham, Chua, Herbison, Olynyk, Trinder: "Liver iron transport." in: World journal of gastroenterology : WJG, Vol. 13, Issue 35, pp. 4725-36, 2007 (PubMed).

Taketani: "Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation." in: The Tohoku journal of experimental medicine, Vol. 205, Issue 4, pp. 297-318, 2005 (PubMed).

Ghosh, Gelman, Maxfield: "Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy." in: Journal of cell science, Vol. 107 ( Pt 8), pp. 2177-89, 1995 (PubMed).

Hansen, Sandvig, van Deurs: "Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification." in: The Journal of cell biology, Vol. 121, Issue 1, pp. 61-72, 1993 (PubMed).

Bártek, Viklický, Fran?k, Angelisová, Dráber, Jarosíková, N?mec, Verlová: "Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity." in: Immunology letters, Vol. 4, Issue 5, pp. 231-5, 1982 (PubMed).

Product cited in: Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Bártek, Viklický, Stratil: "Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding." in: British journal of haematology, Vol. 59, Issue 3, pp. 435-41, 1985 (PubMed).