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Transferrin antibody (Tf) (N-Term)

Details for Product anti-Tf Antibody No. ABIN94486, Supplier: Log in to see
Antigen
  • ltf
  • pro1557
  • pro2086
  • mgc107777
  • LOC692564
  • LOC100144362
  • tf
  • tf-b
  • MGC64306
  • cb285
  • gavi
  • id:ibd3238
  • id:ibd3525
  • sb:cb285
  • wu:fb57g06
  • wu:fb62h02
  • wu:fb63h10
  • wu:fb64h10
  • zgc:112154
  • 143958_at
  • CG6186
  • Dmel\\CG6186
  • TSF1
  • Tf
  • Trf
  • anon-EST:Posey265
  • tsf1
  • TF
  • PRO1557
  • PRO2086
  • TFQTL1
  • AI266983
  • Cd176
  • HP
  • Tfn
  • hpx
  • LTF
  • TFEW
  • conalbumin
Epitope
N-Term
27
9
9
7
6
6
6
5
5
5
5
1
1
1
1
1
1
Reactivity
Human, Pig (Porcine), Rabbit
390
78
64
58
20
13
12
12
9
7
5
4
4
2
2
1
Host
Mouse
319
169
75
33
12
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Transferrin antibody is un-conjugated
45
44
23
9
2
2
2
2
2
2
2
2
2
1
1
1
Application
Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
346
236
117
74
74
70
53
46
30
29
24
22
21
21
19
11
9
8
8
8
6
4
3
2
2
2
1
1
1
1
1
1
Supplier
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Immunogen Purified porcine transferrin.
Clone HTF-14
Isotype IgG1
Specificity The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
No Cross-Reactivity Cow (Bovine), Sheep (Ovine), Dog (Canine), Horse (Equine)
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95 % (by SDS-PAGE)
Alternative Name Transferrin (Tf Antibody Abstract)
Background Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Research Area Cardiovascular, Serum/Plasma Proteins, Enzymes, Metabolism
Application Notes Immunohistochemistry (paraffin sections) Recommended dilution: 10 µg/mL
Positive tissue: placentaImmunocytochemistry
ELISA:
RIA:
Functional Application: The antibody HTF-14 blocks binding of transferrin to the receptor.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for anti-Transferrin antibody (Tf) (N-Term) (ABIN94486) Human transferrin detected by the mouse monoclonal antibody HTF-14. 1. hTransferrin; ...
Product cited in: Nováková, Dráberová, Schürmann et al.: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, 1996 (PubMed).

Bártek, Viklický, Stratil: "Phylogenetically more conservative epitopes among monoclonal antibody-defined antigenic sites of human transferrin are involved in receptor binding." in: British journal of haematology, Vol. 59, Issue 3, pp. 435-41, 1985 (PubMed).

Background publications Steere, Roberts, Byrne et al.: "Properties of a homogeneous C-lobe prepared by introduction of a TEV cleavage site between the lobes of human transferrin." in: Protein expression and purification, 2010 (PubMed).

Graham, Chua, Herbison et al.: "Liver iron transport." in: World journal of gastroenterology : WJG, Vol. 13, Issue 35, pp. 4725-36, 2007 (PubMed).

Taketani: "Aquisition, mobilization and utilization of cellular iron and heme: endless findings and growing evidence of tight regulation." in: The Tohoku journal of experimental medicine, Vol. 205, Issue 4, pp. 297-318, 2005 (PubMed).

Hansen, Sandvig, van Deurs: "Clathrin and HA2 adaptors: effects of potassium depletion, hypertonic medium, and cytosol acidification." in: The Journal of cell biology, Vol. 121, Issue 1, pp. 61-72, 1993 (PubMed).

Ghosh, Gelman, Maxfield: "Quantification of low density lipoprotein and transferrin endocytic sorting HEp2 cells using confocal microscopy." in: Journal of cell science, Vol. 107 ( Pt 8), pp. 2177-89, 1995 (PubMed).

Bártek, Viklický, Fran?k et al.: "Monoclonal antibodies against transferrin. Precipitating mixtures and lack of inter-species cross-reactivity." in: Immunology letters, Vol. 4, Issue 5, pp. 231-5, 1982 (PubMed).