ATP5J antibody (Middle Region)

Catalog No. ABIN950574

The Rabbit Polyclonal anti-ATP5J antibody (ABIN950574) specifically detects ATP5J in WB, IHC (p) and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for ATP5J antibody (Middle Region) (ABIN950574)

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5J antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ATP5J Antibody

Binding Specificity: AA 27-56, Middle Region

Specificity: This antibody reacts to ATPase subunit F6.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 27-56 amino acids from the Central region of human ATP5J

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATP5J

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Alternative Name: ATPase Subunit F6

Background: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.Synonyms: ATP synthase, ATP synthase-coupling factor 6 mitochondrial, ATP5A, ATP5J, ATPM, Complex V subunit F6, F1F0 ATPase subunit F6, H+ transporting, mitochondrial F0 complex, subunit F6

Gene ID: 522

NCBI Accession: NP_001003696

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process