ATP5J antibody (AA 28-56)

Catalog No. ABIN5530313

This Rabbit Polyclonal antibody specifically detects ATP5J in WB, IF and IHC (p). It exhibits reactivity toward Mouse and Rat.

Quick Overview for ATP5J antibody (AA 28-56) (ABIN5530313)

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5J antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATP5J Antibody

Binding Specificity: AA 28-56

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.

Isotype: Ig Fraction

Application Details

Application Notes: For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for ATP5J

Target: ATP5J (ATP Synthase, H+ Transporting, Mitochondrial F0 Complex, Subunit F6 (ATP5J))

Alternative Name: ATP5J

Background: Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.

Molecular Weight: 13 kDa

Gene ID: 522

UniProt: P18859

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process