CD235a/GYPA antibody

Catalog No. ABIN951236

Product Details anti-CD235a/GYPA Antibody

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD235a/GYPA antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: Anti-human Glycophorin A (GpA) Polyclonal Antibody targets the human Glycophorin A protein. This polyclonal antibody recognizes various epitopes on the GpA protein. The 23 residue hydrophobic transmembrane domain has been shown to mediate non-covalent dimerization of the protein under conditions of SDS page (and in other detergents). We also sell a FITC conjugated version of this antibody (AP31671FC-N).

Purification: Affinity chromatography on Protein G

Immunogen: Human Glycophorin A transmembrane protein Donor: Rabbit serum

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: PBS containing 0.02 % sodium azide (NaN3) as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a / GYPA (GYPA Products)

Synonyms: GYPA antibody, gpa antibody, AI853584 antibody, CD235a antibody, GPA antibody, GPErik antibody, GPSAT antibody, HGpMiV antibody, HGpMiXI antibody, HGpSta(C) antibody, MN antibody, MNS antibody, PAS-2 antibody, glycophorin A (MNS blood group) antibody, glycophorin A antibody, GYPA antibody, Gypa antibody

Background: This 131 amino acid, 36 KDa, transmembrane sialoglycoprotein spans the membrane of erythrocytes once. GpA presents its N terminus at the extracellular surface of the human red blood cell, has a single transmembrane domain and an internal cytoplasmic C terminus. GpA contributes to the expression of blood group antigen M and N specificities, which work to prevent red blood cell aggregation in circulation. Studies show that GpA is expressed early on the cell surface during erythropoiesis as pluripotent stem cells are recruited to differentiate into erythrocytes. This makes GpA a useful marker for studying the developmental biology of hematopoietic progenitor cells.Synonyms: GPA, Glycophorin-A, MN sialoglycoprotein, PAS-2, Sialoglycoprotein alpha

Gene ID: 2993

NCBI Accession: NP_002090

Pathways: Maintenance of Protein Location