ATG12 Autophagy Related 12 Homolog (S. Cerevisiae) (ATG12) antibody

Antigen: ATG12 Autophagy Related 12 Homolog (S. Cerevisiae) (ATG12)

Synonyms: APG12, FBR93, APG12L, HAPG12, atg12, CG10861, DmelCG10861, Apg12l, Atg12l, 4931423H11Rik, A330058M13Rik, MGC125080, ATG12, MGC127276, APG12B, AUTOPHAGY 12, AUTOPHAGY 12 B, ATG12A, AUTOPHAGY 12 A, F20D21.38, F20D21_38, DKFZp459D2226

Clonality: Polyclonal

Host: Rabbit

Reactivity: Yeast (Saccharomyces Cerevisiae)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN95224

Additional Information

Alternative name: Apg12

Immunogen: .

Format: Liquid (sterile filtered)

Description: Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. In yeast, autophagy, the delivery of cytoplasmic components to the lysosome/vacuole for degradation, requires a ubiquitin-like protein conjugation system, in which Apg12 is covalently bound to Apg12-Apg5 and Apg16.

Synonyms: APG12, FBR93, APG12L, HAPG12, atg12, CG10861, DmelCG10861, Apg12l, Atg12l, 4931423H11Rik, A330058M13Rik, MGC125080, ATG12, MGC127276, APG12B, AUTOPHAGY 12, AUTOPHAGY 12 B, ATG12A, AUTOPHAGY 12 A, F20D21.38, F20D21_38, DKFZp459D2226

Application Details

Application Notes: Recommended Dilutions: For immunoblotting a 1:2,000 dilution is recommended. A xx kDa band corresponding to yeast APG12 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. For ELISA a 1:1,000 to 1:5,000 dilution is recommended. Researchers should determine optimal titers for other applications.

Concentration: 5.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at 4° C prior to restoration. Restore with 0.1 ml of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.

Research Area: Cancer, Autophagy, Proteolysis / Ubiquitin

Restrictions: For Research Use only

Images

Immunoblot of APG12 fusion protein. Anti-APG12 antibody generated by immunization with recombinant yeast APG12 was tested by immunoblot against yeast lysates expressing the APG12-GFP fusion protein and other UBL fusion proteins. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows positions of free GFP or GFP containing recombinant proteins present in each lysate preparation after reaction with a 1:1,000 dilution of antibodies-online's anti-GFP (code # 600- 101-215) followed by reaction with a 1:15,000 dilution of HRP Donkey-a-Goat IgG MX (code # 605-703-125). Panel C shows specific reaction with APG12 using a 1:2,000 dilution of antibodies-online's IgG fraction of Rabbit-anti-APG12 (Yeast) followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG MX (code # ABIN102010). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4